Spectrophotometer

Determination of the unknown density and absorbance of Cobalt Chloride solution by operating the Spectrophotometer Van Nguyen BIOSC 48, Sec 21453 Date September 11, 2012 mankind We are going to use spectrophotometric techniques by using a spectrophotometer, which footfalls the absorbance by a solution of light of specific wavelength. Also, it allows us to determine the unknown concentration of solution. To do this, we will spud the summation of solution in cuvettes that are placed in the spectrophotometer. Then, spectrophotometer will show the amount of light that is absorbed by the solutes in solution. Spectrophotometry takes advantage of the dual nature of light and merely work with visible light. In this experiment, I was introduced to the spectrophotometry as fashion plate up as how it is used to measure the concentration of compound CoCl2. By seeing the absorbance of CoCl2 at different wavelengths and using the kick wavelength, you than stack use the abso rbance to predict the concentration of unknown tubes. My meditation on the concentration of unknown 2 and unknown 4 were somewhat thirty mg/ml and around ten-spot mg/ml. (I did not do the unknown number 1, 3, and the truly unknown.

I took the answers from my classmate group) Method First, I determined the preoccupancy of CoCl2 by measuring the absorbance of light at different wavelengths. Second, I constructed a standard curve for cobalt chloride; and then install expose the equation of absorption spectrum. Third, using that equation to measure the amount of an unknown concentration of CoCl2. For this exper iment, we could have seven hulky test tube! s, 8 small test tubes; three conelike tubes containing CoCl2 solution, DI water supply, and pipettes. For large tubes, each filled with different concentration of CoCl2 (0, 0.1ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml) plus distilled water (10, 9.9ml, 9.0ml, 8.0ml, 7.0ml, 6.0ml, 5.0ml), and labeled form 0 to 6. The initial tube was containing only if distilled water (10ml) acts as a...If you want to compensate a beneficial essay, order it on our website: OrderCustomPaper.com

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