Study of Conjugation in E.Coli

STUDY OF CONJUGATION IN E.coli AIM : To study the opposition of bacterial conjugation through enthrall of factors,coding for antibiotic resistance. teaching : From one bacterial attain(donor) to another strain(recipient) is a known event that occurs in nature with objective of mixing the factor pool. In otherwise asexually reproducing organism, DNA pitch among bacteria is in different mediating ways: I. Transformation II. Transduction III. Conjugation Conjugation is the most widespread routine of transferring genetic material from one bacterial cubicleular phone to another. It is a process in which unidirectional transfer of DNA is arbitrate by conjugal plasmid DNA or conjugal transposons requiring cell to cell contacts. It was discovered by Lederberg and Tatum. Conjugation is best soundless by considering properties of F-factor, which is a blue circular plasmid DNA, that poop replicate autonomously in the cell or can shuffle into host chromosome and t hus transfer host chromosomal marker. The man-to-man cells with integrated F-factor ar uncaring and allowed to form pure colonies. The resulting straincan transfer chromosomal marker at very high frequency and termed as Hfr strain. INTRODUCTION: The E.coli strain A & B referred to as parental strains.

give A carries an antibiotic loathly gene in F-plasmid coding for tetracycline, piece Strain B is detached of any F-factor, but carries streptomycin patient of gene in its chromosome. consequently both the strains grows on average containing tetracycline or streptomycin to which they are resistant on conjugat ing the ii parental strains, the resulting ! culture go out be resistant to both the antibiotics, i.e. the conjugate sample when plate on a medium containing tetracycline or streptomycin willing survive suggesting that gene transfer has taken place from Strain A to B. that the parental strain when plated on the uniform medium does not survive as they are sore to either tetracycline or streptomycin. MATERIALS REQUIRED:...If you want to experience a skillful essay, order it on our website: OrderCustomPaper.com

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